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4 edition of G protein-coupled receptors cloned from Lymnaea stagnalis found in the catalog.

G protein-coupled receptors cloned from Lymnaea stagnalis

Kim S. Sugamori

G protein-coupled receptors cloned from Lymnaea stagnalis

by Kim S. Sugamori

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Published by National Library of Canada in Ottawa .
Written in English


Edition Notes

Thesis (M.Sc.)--University of Toronto, 1993.

SeriesCanadian theses = Thèses canadiennes
The Physical Object
FormatMicroform
Pagination2 microfiches : negative.
ID Numbers
Open LibraryOL15136707M
ISBN 100315871415
OCLC/WorldCa46534892

Subsequently, one G protein-coupled receptor (GPCR) for oxytocin and three GPCRs for vasopressin (V1a, V1b, and V2) have been cloned and characterized from humans and other mammals (8–13). Vasopressin and oxytocin have a wide range of partially overlapping biological activities. Vasopressin has . Abstract. A cDNA encoding a G-protein-coupled receptor was cloned from the central nervous system of the pond snail Lymnaea stagnalis The predicted amino acid sequence of this cDNA most closely resembles the Drosophila tyramine/octopamine receptor, the Locusta tyramine receptor, and an octopamine receptor (Lym oa 1) that we recently cloned from Lymnaea After stable expression of .

  This text provides a comprehensive overview of recent discoveries and current understandings of G protein-coupled receptors (GPCRs). Recent advances include the first mammalian non-rhodopsin GPCR structures and reconstitution of purified GPCRs into membrane discs for defined studies, novel signaling features including oligomerization, and advances in understanding the . T1 - Functional characterisation of a 5-HT2 receptor cDNA cloned from Lymnaea stagnalis. AU - Gerhardt, C.C. AU - Leysen, J.E.M.F. AU - Planta, R.J. AU - Vreugdenhil, E. AU - van Heerikhuizen, H. PY - Y1 - N2 - A G-protein-coupled receptor (5-HT(2Lym)) resembling members of .

A novel G-protein–coupled receptor (GRL) resembling neuropeptide Y and tachykinin receptors was cloned from the molluscLymnaea stagnalis. Application of a peptide extract from the Lymnaea brain to Xenopus oocytes expressing GRL activated a calcium-dependent chloride channel. The second repeat and the C-terminal part of the Lymnaea receptor are very similar to regions of a specific class of guanine nucleotide-binding protein-coupled receptors, the mammalian.


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G protein-coupled receptors cloned from Lymnaea stagnalis by Kim S. Sugamori Download PDF EPUB FB2

Based on the high homology of genes coding for guanine nucleotide-binding protein (G protein)-coupled receptors, we have cloned a gene for the Lymnaea stagnalis 5-HT (5HTlym) receptor. The putative receptor protein, amino acids long, has highest homology with the Drosophila 5-HT receptors and mammalian 5HT1 by:   A G-protein-coupled receptor (5-HT 2Lym) resembling members of the 5-HT 2 receptor subfamily was cloned from the mollusc Lymnaea nin induces a concentration-dependent increase in intracellular inositol phospates in HEK cells expressing this receptor (EC 50 = nM).

5-HT 2Lym differs from mammalian 5-HT 2 receptors by the presence of a large amino Cited by: Based on the high homology of genes coding for guanine nucleotide-binding protein (G protein)-coupled receptors, we have cloned a gene for the Lymnaea stagnalis 5-HT (5HTlym) receptor.

Cloning, Characterization, and Expression of a G-Protein-Coupled Receptor from Lymnaea stagnalis and Identification of a Leucokinin-Like Peptide, PSFHSWSamide, as Its Endogenous Ligand Kingsley J.

Cox,1 Cornelis P. Tensen,2 Roel C. Van der Schors,4 Ka Wan Li,4 Harm van Heerikhuizen,3 Erno Vreugdenhil,3 Wijnand P. Geraerts,4 and Julian F.

Burke1 1Sussex Centre for Neuroscience. G-protein-coupled receptors as the initial postsynaptic targets. Here we describe the cloning of a neuropeptide receptor from Lymnaea and the isolation of an endogenous ligand.

The cloning and characterization of neuropeptide receptors in Lymnaea thus would be very valuable in further elucidating peptidergic pathways. Indirect evidence suggests that these neuropeptides operate via G-protein-coupled mechanisms indicating the presence of G-protein-coupled receptors as the initial postsynaptic targets.

A novel G-protein–coupled receptor (GRL) resembling neuropeptide Y and tachykinin receptors was cloned from the mollusc Lymnaea stagnalis. Application of a peptide extract from the Lymnaea brain to Xenopus oocytes expressing GRL activated a calcium-dependent chloride channel.

Using this response as a bioassay, we purified the ligand for GRL, Lymnaea cardioexcitatory peptide. Cloning of the Lymnaea Stagnalis P2X Receptor. P2X CODEHOP PCR primers were designed using the CODEHOP algorithm with input blocks generated from predicted extracellular region amino acid sequences (from the end of transmembrane domain 1 to the start of transmembrane domain 2) of the mammalian P2X and available invertebrate P2X receptors using the BlockS WWW server (Fred.

The effects of these neuropeptides are normally mediated via activation of G protein-coupled receptors Alignment of RhoprKR was performed with cloned kinin receptors of invertebrate species W.P.M.

Geraerts, J.F. BurkeCloning, characterization, and expression of a g-protein-coupled receptor from Lymnaea stagnalis and identification.

An intronless gene encoding a putative G protein-coupled receptor was isolated from the genomic library of barnacle Balanus amphitrite Darwin, with probes obtained from degenerate polymerase chain reaction (PCR) primers used to amplify putative transmembrane regions.

The cloned genome DNA specifies an open reading frame of bp encoding amino acids with seven. A novel G protein-coupled receptor mediating both vasopressin and oxytocin-like functions of Lysconopressin in Lymnea stagnalis.

E.R. van Kesteren, C.P. Tensen, A.B. Smit, J. van Minnen, K.S. Kits, W. Meyerhof, We have cloned a receptor, named LSCPR, for vasopressin-related Lys-conopressin in Lymnaea stagnalis.

Degenerate oligonucleotide primers, based on conserved regions of the Lymnaea stagnalis 5-HT 1Lym receptor, were used to amplify G protein-coupled biogenic amine receptor sequences from H.

trivolvis genomic cDNA, resulting in the cloning of two putative serotonin receptors. The deduced gene products both appear to be G protein-coupled serotonin. found, all encoding putative G protein-coupled receptor fragments.

Database searches revealed that one of the cloned cDNAs encoded a protein fragment having a signifi- cantly higher sequence identity with vasopressin and oxy- tocin receptors than with any other G protein-coupled re- ceptor.

A novel G-protein–coupled receptor (GRL) resembling neu-ropeptide Y and tachykinin receptors was cloned from the mollusc Lymnaea stagnalis. Application of a peptide extract from the Lymnaea brain to Xenopus oocytes expressing GRL activated a calcium-dependent chloride channel. Us-ing this response as a bioassay, we purified the ligand for.

tors, wehave cloned a gene for the Lymnaeastagnalis 5-HT (5HTlym)receptor. Theputative receptorprotein, amino acids long, has highest homology with the Drosophila 5-HT receptors. The freshwater snail Lymnaea stagnalis (L.

stagnalis) has served as a successful model for studies in the field of Neuroscience. However, a serious drawback in the molecular analysis of the nervous system of L. stagnalis has been the lack of large-scale genomic or neuronal transcriptome information, thereby limiting the use of this unique model.

In this study, we report 7, distinct EST. Degenerate oligonucleotide primers, based on conserved regions of the Lymnaea stagnalis 5-HT(1Lym) receptor, were used to amplify G protein-coupled biogenic amine receptor sequences from H. Based on the high homology of genes coding for guanine nucleotide-binding protein (G protein)-coupled receptors, we have cloned a gene for the Lymnaea stagnalis 5-HT (5HTlym) receptor.

The putative receptor protein, amino acids long, has highest homology with the Drosophila 5-HT receptors and mammalian 5HT1 receptors.

A cDNA encoding a G-protein-coupled receptor was cloned from the central nervous system of the pond snail Lymnaea stagnalis. The predicted amino acid sequence of this cDNA most closely resembles the Drosophila tyramine/octopamine receptor, the Locusta tyramine receptor, and an octopamine receptor (Lym oa1) that we recently cloned from Lymnaea.

The other cloned octopamine receptors for which a binding analysis has been reported were both from Lymnaea stagnalis. Based on these reported K i s, Lym oa 1 had a fold higher affinity for octopamine compared to tyramine and Lym oa 2 had a fold higher affinity for octopamine compared to tyramine (Gerhardt et al., a, Gerhardt et.

Pituitary adenylate cyclase activating polypeptide (PACAP) and its receptors are present and biochemically active in the central nervous system of the pond snail Lymnaea stagnalis. J Mol Neurosci.

; – /sx [ PubMed ]. Amino acid residues, important for the structure and/or function of G-protein coupled receptors, are also present in the B.

microplus receptor: (i) the sequence, DRY (amino acids –), next to TM 3, apparently couples the receptor to the G-protein (Dixon et al., ); (ii) two cysteines, one found in the first extracellular loop (C Serotonin receptors in Helisoma Kabotyanski et al., ; Morgan et al., ) and Lymnaea stagnalis (Straub and Benjamin, ; Yeoman et al., ).Of the five 5-HT receptors cloned to date from these species, two from.